Strategies for large-scale expansion of clinical-grade human multipotent mesenchymal stromal cells
Por:
Garcia-Fernandez, C, Lopez-Fernandez, A, Borros, S, Lecina, M, Vives, J
Publicada:
15 jul 2020
Resumen:
Production of sufficient numbers of multipotent Mesenchymal Stromal Cells is a great challenge that the industry must address to meet the increasing demand for clinical size product batches. This can be understood as an opportunity to exploit existing bioprocessing technologies already tested successfully in the production of vaccines and biologicals. Herein we analyse the transition from traditional manual cell expansion 2D methodologies to scalable and automated platforms with the capacity to yield billions of cells per batch in a robust, reproducible and cost-effective manner in compliance with current Good Manufacturing Practices. This review gives special relevance to the key features of multiple manufacturing platforms, particularly those using microcarriers to support cell expansion and single-use bioreactors.
Filiaciones:
Garcia-Fernandez, C:
Univ Ramon Llull URL, Inst Quim Sarria, Bioengn Dept, Barcelona, Spain
Lopez-Fernandez, A:
Banc Sang & Teixits, Serv Terapia Cel Lular, Edifici Dr Frederic Duran i Jorda, Barcelona 08005, Spain
Univ Autonoma Barcelona, Vall dHebron Res Inst VHIR, Musculoskeletal Tissue Engn Grp, Passeig Vall dHebron 129-139, Barcelona 08035, Spain
Borros, S:
Univ Ramon Llull URL, Inst Quim Sarria, Bioengn Dept, Barcelona, Spain
Lecina, M:
Univ Ramon Llull URL, Inst Quim Sarria, Bioengn Dept, Barcelona, Spain
Vives, J:
Banc Sang & Teixits, Serv Terapia Cel Lular, Edifici Dr Frederic Duran i Jorda, Barcelona 08005, Spain
Univ Autonoma Barcelona, Vall dHebron Res Inst VHIR, Musculoskeletal Tissue Engn Grp, Passeig Vall dHebron 129-139, Barcelona 08035, Spain
Univ Autonoma Barcelona, Dept Med, Passeig Vall dHebron 129-139, Barcelona 08035, Spain
|