Characterisation of the CTX-M-15-encoding gene in Klebsiella pneumoniae strains from the Barcelona metropolitan area: plasmid diversity and chromosomal integration
Por:
Coelho, A, Gonzalez-Lopez, JJ, Miro, E, Alonso-Tarres, C, Mirelis, B, Larrosa, MN, Bartolome, RM, Andreu, A, Navarro, F, Johnson, JR, Prats, G
Publicada:
1 jul 2010
Resumen:
The localisation and genetic organisation of bla(CTX-M-15) were studied in 37 CTX-M-15-producing Klebsiella pneumoniae isolates collected from 2005 to 2008 within the Barcelona metropolitan area. Polymerase chain reaction (PCR)-based replicon typing and Southern hybridisations were used to identify the bla(CTX-M-15) location. The genetic environment was analysed by PCR mapping and sequencing, and transferability of bla(CTX-M-15) was evaluated by conjugation and transformation assays. The majority of the 37 isolates carried bla(CTX-M-15) in a plasmid location, frequently associated with the aac(6')-Ib-cr gene. Plasmids encoding bla(CTX-M-15) carried three distinct replicons, i.e. IncFII, IncR and IncFIIk, the latter two not having been described previously in association with bla(CTX-M-15). Several of these plasmids were not self-transferable. Furthermore, in all isolates belonging to sequence type ST-1, bla(CTX-M-15) was found integrated into the K. pneumoniae chromosome. In all the studied isolates, the mobile element ISEcp1 was found upstream of bla(CTX-M-15), whereas IS26 was found inserted within ISEcp1 in several isolates, in previously unreported positions. In conclusion, these findings indicate that among K. pneumoniae strains isolated in the Barcelona metropolitan area, bla(CTX-M-15) is associated with diverse genetic elements, including the IncR and IncFIIk replicons, as reported for the first time here, and the chromosome. (C) 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Filiaciones:
Coelho, A:
Hosp Gen Valle Hebron, Microbiol Serv, Barcelona 08035, Spain
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
Gonzalez-Lopez, JJ:
Hosp Gen Valle Hebron, Microbiol Serv, Barcelona 08035, Spain
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
Miro, E:
Hosp Santa Creu & Sant Pau, Microbiol Serv, Barcelona, Spain
Alonso-Tarres, C:
Hosp Gen Hospitalet, Microbiol Serv, Barcelona, Spain
Mirelis, B:
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
Hosp Santa Creu & Sant Pau, Microbiol Serv, Barcelona, Spain
Larrosa, MN:
Hosp Gen Valle Hebron, Microbiol Serv, Barcelona 08035, Spain
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
Bartolome, RM:
Hosp Gen Valle Hebron, Microbiol Serv, Barcelona 08035, Spain
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
Andreu, A:
Hosp Gen Valle Hebron, Microbiol Serv, Barcelona 08035, Spain
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
Navarro, F:
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
Hosp Santa Creu & Sant Pau, Microbiol Serv, Barcelona, Spain
Johnson, JR:
Vet Affairs Med Ctr, Minneapolis, MN USA
Univ Minnesota, Minneapolis, MN USA
Prats, G:
Hosp Gen Valle Hebron, Microbiol Serv, Barcelona 08035, Spain
Univ Autonoma Barcelona, E-08193 Barcelona, Spain
Green Accepted
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