Proteomic analysis of electronegative low-density lipoprotein
Por:
Bancells, C, Canals, F, Benitez, S, Colome, N, Julve, J, Ordonez-Llanos, J, Sanchez-Quesada, JL
Publicada:
1 dic 2010
Resumen:
Low density lipoprotein is a heterogeneous group of lipoproteins that differs in lipid and protein composition. One copy of apolipoprotein (apo) B accounts for over 95% of the LDL protein, but the presence of minor proteins could disturb its biological behavior. Our aim was to study the content of minor proteins in LDL subfractions separated by anion exchange chromatography. Electropositive LDL [LDL(+)] is the native form, whereas electronegative LDL [LDL(-)] is a minor atherogenic fraction present in blood. LC-ESI MS/MS analysis of both LDL fractions identified up to 28 different proteins. Of these, 13 proteins, including apoB, were detected in all the analyzed samples. LDL(-) showed a higher content of most minor proteins. Statistical analysis of proteomic data indicated that the content of apoE, apoA-I, apoC-III, apoA-II, apoD, apoF, and apoJ was higher in LDL(-) than in LDL(+). Immunoturbidimetry, ELISA, or Western blot analysis confirmed these differences. ApoJ and apoF presented the highest difference between LDL(+) and LDL(-) (>15-fold).jlr In summary, the increased content of several apolipoproteins, and specifically of apoF and apoJ, could be related to the physicochemical characteristics of LDL(-), such as apoB misfolding, aggregation, and abnormal lipid composition.-Bancells, C., F. Canals, S. Benitez, N. Colome, J. Julve, J. Ordonez-Llanos, and J. L. Sanchez-Quesada. Proteomic analysis of electronegative low-density lipoprotein. J. Lipid Res. 2010. 51: 3508-3515.
Filiaciones:
Bancells, C:
Hosp Santa Creu & Sant Pau, Inst Recerca, Serv Bioquim, Barcelona, Spain
Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, E-08193 Barcelona, Spain
Canals, F:
Hosp Univ Vall Hebron, Inst Recerca, Lab Prote, Barcelona, Spain
Benitez, S:
Hosp Santa Creu & Sant Pau, Inst Recerca, Serv Bioquim, Barcelona, Spain
Colome, N:
Hosp Univ Vall Hebron, Inst Recerca, Lab Prote, Barcelona, Spain
Julve, J:
Hosp Santa Creu & Sant Pau, Inst Recerca, Serv Bioquim, Barcelona, Spain
CIBER Diabet & Enfermedades Metab Asociadas CIBER, Barcelona, Spain
Ordonez-Llanos, J:
Hosp Santa Creu & Sant Pau, Inst Recerca, Serv Bioquim, Barcelona, Spain
Univ Autonoma Barcelona, Dept Bioquim & Biol Mol, E-08193 Barcelona, Spain
Sanchez-Quesada, JL:
Hosp Santa Creu & Sant Pau, Inst Recerca, Serv Bioquim, Barcelona, Spain
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