Dehydroepiandrosterone reverses chronic hypoxia/reoxygenation-induced right ventricular dysfunction in rats
Por:
de la Roque, ED, Bellance, N, Rossignol, R, Begueret, H, Billaud, M, Dos Santos, P, Ducret, T, Marthan, R, Dahan, D, Ramos-Barbon, D, Amor-Carro, O, Savineau, JP, Fayon, M
Publicada:
1 dic 2012
Resumen:
Dehydroepiandrosterone (DHEA) prevents chronic hypoxia-induced pulmonary hypertension and associated right ventricle dysfunction in rats. In this animal model, reoxygenation following hypoxia reverses pulmonary hypertension but not right ventricle dysfunction. We thus studied the effect of DHEA on the right ventricle after reoxygenation, i.e. after a normoxic recovery phase secondary to chronic hypoxia in rats. Right ventricle function was assessed in vivo by Doppler echocardiography and in vitro by the isolated perfused heart technique in three groups of animals: control, recovery (21 days of hypoxia followed by 21 days of normoxia) and recovery DHEA (30 mg.kg(-1) every 2 days during the recovery phase).
Right ventricle tissue was assessed by optical and electron microscopy. DHEA abolished right ventricle diastolic dysfunction, as the echographic E wave remained close to that of controls (mean +/- SD 76.5 +/- 2.4 and 79.7 +/- 1.7 cm.s(-1), respectively), whereas it was diminished to 40.3 +/- 3.7 in the recovery group.
DHEA also abolished right ventricle systolic dysfunction, as shown by the inhibition of the increase in the slope of the pressure-volume curve in isolated heart. The DHEA effect was related to cardiac myocytes proliferation.
In conclusion, DHEA prevents right ventricle dysfunction in this animal model by preventing cardiomyocyte alteration.
Filiaciones:
de la Roque, ED:
CHU Bordeaux, Hop Pellegrin Enfants, F-33076 Bordeaux, France
Univ Bordeaux, Ctr Rech Cardiothorac, U1045, Bordeaux, France
INSERM, U1045, U688, Bordeaux, France
INSERM, U1034, Bordeaux, France
Bellance, N:
INSERM, U1045, U688, Bordeaux, France
INSERM, U1034, Bordeaux, France
Rossignol, R:
INSERM, U1045, U688, Bordeaux, France
INSERM, U1034, Bordeaux, France
Begueret, H:
Univ Bordeaux, Ctr Rech Cardiothorac, U1045, Bordeaux, France
INSERM, U1045, U688, Bordeaux, France
INSERM, U1034, Bordeaux, France
Billaud, M:
Univ Bordeaux, Ctr Rech Cardiothorac, U1045, Bordeaux, France
INSERM, U1045, U688, Bordeaux, France
INSERM, U1034, Bordeaux, France
Dos Santos, P:
INSERM, U1045, U688, Bordeaux, France
INSERM, U1034, Bordeaux, France
Ducret, T:
Univ Bordeaux, Ctr Rech Cardiothorac, U1045, Bordeaux, France
INSERM, U1045, U688, Bordeaux, France
INSERM, U1034, Bordeaux, France
Marthan, R:
Univ Bordeaux, Ctr Rech Cardiothorac, U1045, Bordeaux, France
INSERM, U1045, U688, Bordeaux, France
INSERM, U1034, Bordeaux, France
Dahan, D:
Univ Bordeaux, Ctr Rech Cardiothorac, U1045, Bordeaux, France
INSERM, U1045, U688, Bordeaux, France
INSERM, U1034, Bordeaux, France
Ramos-Barbon, D:
Univ Autonoma Barcelona, Resp Dept, Hosp Santa Creu & St Pau, E-08193 Barcelona, Spain
Amor-Carro, O:
Univ Autonoma Barcelona, Resp Dept, Hosp Santa Creu & St Pau, E-08193 Barcelona, Spain
Savineau, JP:
Univ Bordeaux, Ctr Rech Cardiothorac, U1045, Bordeaux, France
INSERM, U1045, U688, Bordeaux, France
INSERM, U1034, Bordeaux, France
Fayon, M:
Univ Bordeaux, Ctr Rech Cardiothorac, U1045, Bordeaux, France
INSERM, U1045, U688, Bordeaux, France
INSERM, U1034, Bordeaux, France
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