Phytosterol-mediated inhibition of intestinal cholesterol absorption in mice is independent of liver X receptor
Por:
Cedó L., Santos D., Ludwig I.A., Silvennoinen R., García-León A., Kaipiainen L., Carbó J.M., Valledor A.F., Gylling H., Motilva M.-J., Kovanen P.T., Lee-Rueckert M., Blanco-Vaca F., Escolà-Gil J.C.
Publicada:
1 ene 2017
Resumen:
Scope: Previous studies have proposed that phytosterols activate liver X receptors (LXR) in the intestine, thereby reducing intestinal cholesterol absorption and promoting fecal cholesterol excretion. Methods and results: In the present study, we examined the effects of dietary phytosterol supplementation on intestinal cholesterol absorption and fecal neutral sterol excretion in LXRaß-deficient mice, and wild-type mice treated with synthetic high-affinity LXRaß agonists. LXRaß deficiency led to an induction of intestinal cholesterol absorption and liver cholesterol accumulation. Phytosterol feeding resulted in an approximately 40% reduction of intestinal cholesterol absorption both in wild-type and LXRaß-deficient mice, reduced dietary cholesterol accumulation in liver and promoted the excretion of fecal cholesterol-derived compounds. Furthermore, phytosterols produced additive inhibitory effects on cholesterol absorption in mice treated with LXRaß agonists. Conclusions: Our data confirm the effect of LXR in regulating intestinal cholesterol absorption and demonstrate that the cholesterol-lowering effects of phytosterols occur in an LXR-independent manner. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Filiaciones:
Cedó L.:
Institut d'Investigacions Biomèdiques (IIB) Sant PauBarcelona, Spain
CIBER de Diabetes y Enfermedades Metabólicas Asociadas, CIBERDEM, Hospitalet de Llobregat, Spain
Santos D.:
Institut d'Investigacions Biomèdiques (IIB) Sant PauBarcelona, Spain
CIBER de Diabetes y Enfermedades Metabólicas Asociadas, CIBERDEM, Hospitalet de Llobregat, Spain
Ludwig I.A.:
Food Technology Department, UTPV-XaRTA, Agrotecnio Center, University of LleidaLleida, Spain
Silvennoinen R.:
Wihuri Research Institute, Helsinki, Finland
García-León A.:
Institut d'Investigacions Biomèdiques (IIB) Sant PauBarcelona, Spain
Departament de Bioquímica, Biología Molecular i Biomedicina, Universitat Autònoma de BarcelonaBarcelona, Spain
Kaipiainen L.:
University of Helsinki and Helsinki University Central Hospital, Department of Internal Medicine, Helsinki, Finland
Carbó J.M.:
Department of Cellular Biology, Physiology and Immunology, School of Biology, University of BarcelonaBarcelona, Spain
Valledor A.F.:
Department of Cellular Biology, Physiology and Immunology, School of Biology, University of BarcelonaBarcelona, Spain
Gylling H.:
University of Helsinki and Helsinki University Central Hospital, Department of Internal Medicine, Helsinki, Finland
Motilva M.-J.:
Food Technology Department, UTPV-XaRTA, Agrotecnio Center, University of LleidaLleida, Spain
Kovanen P.T.:
Wihuri Research Institute, Helsinki, Finland
Lee-Rueckert M.:
Wihuri Research Institute, Helsinki, Finland
Blanco-Vaca F.:
Institut d'Investigacions Biomèdiques (IIB) Sant PauBarcelona, Spain
CIBER de Diabetes y Enfermedades Metabólicas Asociadas, CIBERDEM, Hospitalet de Llobregat, Spain
Departament de Bioquímica, Biología Molecular i Biomedicina, Universitat Autònoma de BarcelonaBarcelona, Spain
Escolà-Gil J.C.:
Institut d'Investigacions Biomèdiques (IIB) Sant PauBarcelona, Spain
CIBER de Diabetes y Enfermedades Metabólicas Asociadas, CIBERDEM, Hospitalet de Llobregat, Spain
Departament de Bioquímica, Biología Molecular i Biomedicina, Universitat Autònoma de BarcelonaBarcelona, Spain
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