The induction of cytokine release in monocytes by electronegative low-density lipoprotein (LDL) is related to its higher ceramide content than native LDL
Por:
Estruch M., Sanchez-Quesada J.L., Beloki L., Ordoñez-Llanos J., Benitez S.
Publicada:
1 ene 2013
Resumen:
Electronegative low-density lipoprotein (LDL(-)) is a minor modified LDL subfraction that is present in blood. LDL(-) promotes inflammation and is associated with the development of atherosclerosis. We previously reported that the increase of cytokine release promoted by this lipoprotein subfraction in monocytes is counteracted by high-density lipoprotein (HDL). HDL also inhibits a phospholipase C-like activity (PLC-like) intrinsic to LDL(-). The aim of this work was to assess whether the inhibition of the PLC-like activity by HDL could decrease the content of ceramide (CER) and diacylglycerol (DAG) generated in LDL(-). This knowledge would allow us to establish a relationship between these compounds and the inflammatory activity of LDL(-). LDL(-) incubated at 37 °C for 20 h increased its PLC-like activity and, subsequently, the amount of CER and DAG. We found that incubating LDL(-) with HDL decreased both products in LDL(-). Native LDL was modified by lipolysis with PLC or by incubation with CER-enriched or DAG-enriched liposomes. The increase of CER in native LDL significantly increased cytokine release, whereas the enrichment in DAG did not show these inflammatory properties. These data point to CER, a resultant product of the PLC-like activity, as a major determinant of the inflammatory activity induced by LDL(-) in monocytes. © 2013 by the authors; licensee MDPI, Basel, Switzerland.
Filiaciones:
Estruch M.:
Cardiovascular Biochemistry Group, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona 08041, Spain
Biochemistry and Molecular Biology Department, Autonomous University of Barcelona, Sant Pau Hospital Unity, Barcelona 08041, Spain
Sanchez-Quesada J.L.:
Cardiovascular Biochemistry Group, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona 08041, Spain
Beloki L.:
Biochemistry and Molecular Biology Department, Autonomous University of Barcelona, Sant Pau Hospital Unity, Barcelona 08041, Spain
Ordoñez-Llanos J.:
Cardiovascular Biochemistry Group, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona 08041, Spain
Biochemistry and Molecular Biology Department, Autonomous University of Barcelona, Sant Pau Hospital Unity, Barcelona 08041, Spain
Biochemistry Department, Sant Pau Hospital, Barcelona 08041, Spain
Benitez S.:
Cardiovascular Biochemistry Group, Biomedical Research Institute Sant Pau (IIB Sant Pau), Barcelona 08041, Spain
All Open Access, Gold
|