Phytosterols and omega 3 supplementation exert novel regulatory effects on metabolic and inflammatory pathways: A proteomic study
Por:
Lambert C., Cubedo J., Padró T., Sánchez-Hernández J., Antonijoan R.M., Perez A., Badimon L.
Publicada:
1 ene 2017
Resumen:
Cardiovascular disease (CVD) remains one of the major causes of death and disability worldwide. In addition to drug treatment, nutritional interventions or supplementations are becoming a health strategy for CVD prevention. Phytosterols (PhyS) are natural components that have been shown to reduce cholesterol levels; while poly-unsaturated fatty acids (PUFA), mainly omega-3 (?3) fatty acids, have shown to reduce triglyceride levels. Here we aimed to investigate whether the proteins in the main lipoproteins (low density lipoproteins (LDL) and high density lipoproteins (HDL)) as well as proteins in the lipid free plasma fraction (LPDP) were regulated by the intake of PhyS-milk or ?3-milk, in overweight healthy volunteers by a proteomic based systems biology approach. The study was a longitudinal crossover trial, including thirty-two healthy volunteers with body mass index (BMI) 25–35 kg/m2 (Clinical Trial: ISRCTN78753338). Basal samples before any intervention and after 4 weeks of intake of PhyS or ?3-milk were analyzed. Proteomic profiling by two dimensional electrophoresis (2-DE) followed by mass spectrometry-(MALDI/TOF), ELISA, Western blot, conventional biochemical analysis, and in-silico bioinformatics were performed. The intake of PhyS-milk did not induce changes in the lipid associated plasma protein fraction, whereas ?3-milk significantly increased apolipoprotein (Apo)- E LDL content (p = 0.043) and induced a coordinated increase in several HDL-associated proteins, Apo A-I, lecitin cholesterol acyltransferase (LCAT), paraoxonase-1 (PON-1), Apo D, and Apo L1 (p < 0.05 for all). Interestingly, PhyS-milk intake induced a reduction in inflammatory molecules not seen after ?3-milk intake. Serum amyloid P component (SAP) was reduced in the LPDP protein fraction (p = 0.001) of subjects taking PhyS-milk and C-C motif chemokine 2 (CCL2)expression detected by reverse transcription polymerase chain reaction (RT-PCR) analysis in white blood cells was significantly reduced (p = 0.013). No changes were observed in the lipid-free plasma proteome with ?3-milk. Our study provides novel results and highlights that the PhyS-milk induces attenuation of the pro-inflammatory pathways, whereas ?3-milk induces improvement in lipid metabolic pathways. © 2017 by the authors. Licensee MDPI, Basel, Switzerland..
Filiaciones:
Lambert C.:
Cardiovascular Science Institute—ICCC IIB-Sant Pau, Barcelona, 08025, Spain
Cubedo J.:
Cardiovascular Science Institute—ICCC IIB-Sant Pau, Barcelona, 08025, Spain
Ciber CV, Madrid, 28029, Spain
Padró T.:
Cardiovascular Science Institute—ICCC IIB-Sant Pau, Barcelona, 08025, Spain
Ciber CV, Madrid, 28029, Spain
Sánchez-Hernández J.:
Ciber DEM, Madrid, 28029, Spain
Endocrinology Department, Hospital Sant Pau, IIB-Sant Pau, Barcelona, 08025, Spain
Antonijoan R.M.:
Medicament ResearchCenter (CIM), Hospital Sant Pau, IIB-Sant Pau, Barcelona, 08025, Spain
Perez A.:
Endocrinology Department, Hospital Sant Pau, IIB-Sant Pau, Barcelona, 08025, Spain
Badimon L.:
Cardiovascular Science Institute—ICCC IIB-Sant Pau, Barcelona, 08025, Spain
Ciber CV, Madrid, 28029, Spain
Cardiovascular Research Chair UAB, Barcelona, 08025, Spain
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