Prevalence of SXT/R391-like integrative and conjugative elements carrying blaC MY-2 in Proteus mirabilis
Por:
Mata C., Navarro F., Miró E., Walsh T.R., Mirelis B., Toleman M.
Publicada:
1 ene 2011
Resumen:
Objectives: To characterize the vectors involved in the dissemination of bla CMY-2 genes in clinical isolates of Proteus mirabilis collected between 1999 and 2007. Methods: Plasmid analysis of 19 P. mirabilis carrying ampC genes was performed by PCR-based replicon typing, S1-PFGE and Southern hybridization with ampC and replicon probes. Isolates that could not be characterized were examined for the presence of SXT/R391-like elements. To demonstrate the involvement of these elements in the dissemination of bla CMY-2, we performed a PCR amplification of the integrase (int) and toxin/antitoxin (TA) genes from SXT/R391-like integrative conjugative elements (ICEs). Later on, I-Ceu-I PFGE gels and hybridization with bla CMY-2, int and prfC probes were performed. The genetic organization of bla CMY-2 was also studied. Results: ampC genes were located on large conjugative plasmids in 11 of the 19 (58%) P. mirabilis studied. However, in eight of these isolates a plasmid was not involved in the mobilization of ampC genes. I-Ceu-I PFGE and hybridization analyses revealed thatbla CMY-2 were chromosomally located in these eight P. mirabilis isolates. The genetic organization of bla CMY-2 and hybridization analyses revealed that bla CMY-2 was carried by an ICE almost identical to ICEPmiJpan1 in seven out of these eight isolates. Conclusions: The prevalence of ICEs carrying bla CMY-2 was surprisingly high [37% (7 out of 19)]. This is the first study giving prevalence data on ICEs carrying bla CMY-2 genes. These results suggest the need to study these mobile genetic elements in the context of dissemination of acquired AmpC ß-lactamases and also of other ß-lactamases, such as extended-spectrum ß-lactamases and carbapenemases. © The Author 2011. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.
Filiaciones:
Mata C.:
Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Universitat Autónoma de Barcelona, Institut d'Investigacions Biomédiques Sant Pau, Barcelona, Spain
Navarro F.:
Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Universitat Autónoma de Barcelona, Institut d'Investigacions Biomédiques Sant Pau, Barcelona, Spain
Miró E.:
Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Universitat Autónoma de Barcelona, Institut d'Investigacions Biomédiques Sant Pau, Barcelona, Spain
Walsh T.R.:
Section of Medical Microbiology, IIB, School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom
Mirelis B.:
Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, Universitat Autónoma de Barcelona, Institut d'Investigacions Biomédiques Sant Pau, Barcelona, Spain
Toleman M.:
Section of Medical Microbiology, IIB, School of Medicine, Cardiff University, Heath Park, Cardiff, United Kingdom
Bronze, Green Published, All Open Access, Green
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