Activity of Antibiotics and Potential Antibiofilm Agents against Biofilm-Producing Mycobacterium avium-intracellulare Complex Causing Chronic Pulmonary Infections
Por:
Portell-Buj E., González-Criollo C., López-Gavín A., Fernández-Pittol M., Busquets M.A., Estelrich J., Garrigó M., Rubio M., Tudó G., Gonzalez-Martin J.
Publicada:
1 may 2022
Resumen:
Nontuberculous mycobacteria (NTM) cause lung infections in patients with underlying pulmonary diseases (PD). The Mycobacterium avium-intracellulare complex (MAC) is the most frequently involved NTM. The MAC-PD treatment is based on the administration of several antibiotics for long periods of time. Nonetheless, treatment outcomes remain very poor. Among the factors involved is the ability of MAC isolates to form biofilm. The aim of the study was to assess the in vitro activity of different antibiotics and potential antibiofilm agents (PAAs) against MAC biofilm. Four antibiotics and six PAAs, alone and/or in combination, were tested against planktonic forms of 11 MAC clinical isolates. Biofilm was produced after 4 weeks of incubation and analyzed with the crystal violet assay. The antibiotics and PAAs were tested by measuring the absorbance (minimum biofilm inhibition concentrations, MBICs) and by performing subcultures (minimum biofilm eradication concentrations, MBECs). The clarithromycin/amikacin and clarithromycin/ethambutol combinations were synergistic, decreasing the MBECs values compared to the individual antibiotics. The amikacin/moxifloxacin combination showed indifference. The MBIC values decreased significantly when PAAs were added to the antibiotic combinations. These results suggest that antibiotic combinations should be further studied to establish their antibiofilm activity. Moreover, PAAs could act against the biofilm matrix, facilitating the activity of antibiotics.
Filiaciones:
Portell-Buj E.:
Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, c/Casanova 143, Barcelona, 08036, Spain
ISGlobal Barcelona, Institute for Global Health, c/Rosselló 132, Barcelona, 08036, Spain
Servei de Microbiologia, CDB, Hospital Clínic de Barcelona, c/Villarroel 170, Barcelona, 08036, Spain
González-Criollo C.:
Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, c/Casanova 143, Barcelona, 08036, Spain
Unidad de Investigación en Biomedicina, Zurita&Zurita Laboratorios, Quito, 170104, Ecuador
López-Gavín A.:
Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, c/Casanova 143, Barcelona, 08036, Spain
ISGlobal Barcelona, Institute for Global Health, c/Rosselló 132, Barcelona, 08036, Spain
Servei de Microbiologia, CDB, Hospital Clínic de Barcelona, c/Villarroel 170, Barcelona, 08036, Spain
Fernández-Pittol M.:
Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, c/Casanova 143, Barcelona, 08036, Spain
ISGlobal Barcelona, Institute for Global Health, c/Rosselló 132, Barcelona, 08036, Spain
Servei de Microbiologia, CDB, Hospital Clínic de Barcelona, c/Villarroel 170, Barcelona, 08036, Spain
Busquets M.A.:
Department de Farmàcia, Tecnologia Farmacèutica i Físicoquímica, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, Av. Joan XXIII, 27-31, Barcelona, 08028, Spain
Institut de Nanociència i Nanotecnologia, IN2UB, Facultat de Química, Av. Diagonal 645, Barcelona, 08028, Spain
Estelrich J.:
Department de Farmàcia, Tecnologia Farmacèutica i Físicoquímica, Facultat de Farmàcia i Ciències de l’Alimentació, Universitat de Barcelona, Av. Joan XXIII, 27-31, Barcelona, 08028, Spain
Institut de Nanociència i Nanotecnologia, IN2UB, Facultat de Química, Av. Diagonal 645, Barcelona, 08028, Spain
Garrigó M.:
Servei de Microbiologia, Fundació de Gestió de l’Hospital de la Santa Creu i Sant Pau, c/Sant Quintí 89, Barcelona, 08026, Spain
Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau), c/Sant Quintí 77, Barcelona, 08041, Spain
Rubio M.:
Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau), c/Sant Quintí 77, Barcelona, 08041, Spain
Tudó G.:
Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, c/Casanova 143, Barcelona, 08036, Spain
ISGlobal Barcelona, Institute for Global Health, c/Rosselló 132, Barcelona, 08036, Spain
Servei de Microbiologia, CDB, Hospital Clínic de Barcelona, c/Villarroel 170, Barcelona, 08036, Spain
Gonzalez-Martin J.:
Departament de Fonaments Clínics, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, c/Casanova 143, Barcelona, 08036, Spain
ISGlobal Barcelona, Institute for Global Health, c/Rosselló 132, Barcelona, 08036, Spain
Servei de Microbiologia, CDB, Hospital Clínic de Barcelona, c/Villarroel 170, Barcelona, 08036, Spain
CIBER of Infectiuos Diseases (CIBERINFEC), Instituto de Salud Carlos III, Madrid, 28029, Spain
gold, Green Published, All Open Access, Gold, Green
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