Fluorescent dye labeling changes the biodistribution of tumor-targeted nanoparticles
Por:
Álamo P., Pallarès V., Céspedes M.V., Falgàs A., Sanchez J.M., Serna N., Sánchez-garcía L., Voltà-duràn E., Morris G.A., Sánchez-chardi A., Casanova I., Mangues R., Vazquez E., Villaverde A., Unzueta U.
Publicada:
1 ene 2020
Resumen:
Fluorescent dye labeling is a common strategy to analyze the fate of administered nanoparticles in living organisms. However, to which extent the labeling processes can alter the original nanoparticle biodistribution has been so far neglected. In this work, two widely used fluorescent dye molecules, namely, ATTO488 (ATTO) and Sulfo-Cy5 (S-Cy5), have been covalently attached to a well-characterized CXCR4-targeted self-assembling protein nanoparticle (known as T22-GFP-H6). The biodistribution of labeled T22-GFP-H6-ATTO and T22-GFP-H6-S-Cy5 nanoparticles has been then compared to that of the non-labeled nanoparticle in different CXCR4+ tumor mouse models. We observed that while parental T22-GFP-H6 nanoparticles accumulated mostly and specifically in CXCR4+ tumor cells, labeled T22-GFP-H6-ATTO and T22-GFP-H6-S-Cy5 nanoparticles showed a dramatic change in the biodistribution pattern, accumulating in non-target organs such as liver or kidney while reducing tumor targeting capacity. Therefore, the use of such labeling molecules should be avoided in target and non-target tissue uptake studies during the design and development of targeted nanoscale drug delivery systems, since their effect over the fate of the nanomaterial can lead to considerable miss-interpretations of the actual nanoparticle biodistribution. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Filiaciones:
Álamo P.:
Biomedical Research Institute Sant Pau (IIB Sant Pau), Sant Antoni Mª Claret 167, Barcelona, 08025, Spain
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Josep Carreras Leukaemia Research Institute (IJC Campus Sant Pau), Barcelona, 08025, Spain
Pallarès V.:
Biomedical Research Institute Sant Pau (IIB Sant Pau), Sant Antoni Mª Claret 167, Barcelona, 08025, Spain
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Josep Carreras Leukaemia Research Institute (IJC Campus Sant Pau), Barcelona, 08025, Spain
Céspedes M.V.:
Biomedical Research Institute Sant Pau (IIB Sant Pau), Sant Antoni Mª Claret 167, Barcelona, 08025, Spain
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Falgàs A.:
Biomedical Research Institute Sant Pau (IIB Sant Pau), Sant Antoni Mª Claret 167, Barcelona, 08025, Spain
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Josep Carreras Leukaemia Research Institute (IJC Campus Sant Pau), Barcelona, 08025, Spain
Sanchez J.M.:
Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
ICTA & Cátedra de Química Biológica, Departamento de Química, Instituto de Investigaciones Biológicas y Tecnológicas (IIBYT) (CONICET—Universidad Nacional de Córdoba), FCEFyN, UNC, Av. Velez Sarsfield 1611, Córdoba, X 5016GCA, Argentina
Serna N.:
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Sánchez-garcía L.:
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Voltà-duràn E.:
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Morris G.A.:
Department of Chemical Sciences, School of Applied Science, University of Huddersfield, Queensgate, Huddersfield, HD1 3DH, United Kingdom
Sánchez-chardi A.:
Servei de Microscòpia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Departament de Biologia Evolutiva, Ecologia i Ciències Ambientals, Facultat de Biologia, Universitat de Barcelona, Barcelona, 08028, Spain
Casanova I.:
Biomedical Research Institute Sant Pau (IIB Sant Pau), Sant Antoni Mª Claret 167, Barcelona, 08025, Spain
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Josep Carreras Leukaemia Research Institute (IJC Campus Sant Pau), Barcelona, 08025, Spain
Mangues R.:
Biomedical Research Institute Sant Pau (IIB Sant Pau), Sant Antoni Mª Claret 167, Barcelona, 08025, Spain
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Josep Carreras Leukaemia Research Institute (IJC Campus Sant Pau), Barcelona, 08025, Spain
Vazquez E.:
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Villaverde A.:
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Institut de Biotecnologia i de Biomedicina, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Unzueta U.:
Biomedical Research Institute Sant Pau (IIB Sant Pau), Sant Antoni Mª Claret 167, Barcelona, 08025, Spain
CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), C/Monforte de Lemos 3–5, Madrid, 28029, Spain
Josep Carreras Leukaemia Research Institute (IJC Campus Sant Pau), Barcelona, 08025, Spain
Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193, Spain
Green Published, gold, All Open Access, Gold, Green
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