A major role for RCAN1 in atherosclerosis progression
Por:
Mendez-Barbero, N, Esteban, V, Villahoz, S, Escolano, A, Urso, K, Alfranca, A, Rodriguez, C, Sanchez, SA, Osawa, T, Andres, V, Martinez-Gonzalez, J, Minami, T, Redondo, JM, Campanero, MR
Publicada:
1 dic 2013
Resumen:
Atherosclerosis is a complex inflammatory disease involving extensive vascular vessel remodelling and migration of vascular cells. As RCAN1 is implicated in cell migration, we investigated its contribution to atherosclerosis. We show RCAN1 induction in atherosclerotic human and mouse tissues. Rcan1 was expressed in lesional macrophages, endothelial cells and vascular smooth muscle cells and was induced by treatment of these cells with oxidized LDLs (oxLDLs). Rcan1 regulates CD36 expression and its genetic inactivation reduced atherosclerosis extension and severity in Apoe-/- mice. This effect was mechanistically linked to diminished oxLDL uptake, resistance to oxLDL-mediated inhibition of macrophage migration and increased lesional IL-10 and mannose receptor expression. Moreover, Apoe-/-Rcan1-/- macrophages expressed higher-than-Apoe-/- levels of anti-inflammatory markers. We previously showed that Rcan1 mediates aneurysm development and that its expression is not required in haematopoietic cells for this process. However, transplantation of Apoe-/-Rcan1-/- bone-marrow (BM) cells into Apoe-/- recipients confers atherosclerosis resistance. Our data define a major role for haematopoietic Rcan1 in atherosclerosis and suggest that therapies aimed at inhibiting RCAN1 expression or function might significantly reduce atherosclerosis burden. © 2013 The Authors. Published by John Wiley and Sons, Ltd on behalf of EMBO.
Filiaciones:
Mendez-Barbero, N:
Ctr Nacl Invest Cardiovasc, Dept Vasc Biol & Inflammat, Madrid, Spain
Esteban, V:
Ctr Nacl Invest Cardiovasc, Dept Vasc Biol & Inflammat, Madrid, Spain
Villahoz, S:
Ctr Nacl Invest Cardiovasc, Dept Vasc Biol & Inflammat, Madrid, Spain
Escolano, A:
Ctr Nacl Invest Cardiovasc, Dept Vasc Biol & Inflammat, Madrid, Spain
Urso, K:
Ctr Nacl Invest Cardiovasc, Dept Vasc Biol & Inflammat, Madrid, Spain
Alfranca, A:
Carlos III Hlth Inst, Inst Rare Dis Res, Dept Human Genet, Madrid, Spain
Rodriguez, C:
IIB Sant Pau, Ctr Invest Cardiovasc CSIC ICCC, Barcelona, Spain
Sanchez, SA:
Ctr Nacl Invest Cardiovasc, Microscopy & Dynam Imaging Unit, Madrid, Spain
Osawa, T:
Univ Tokyo, RCAST, Div Vasc Biol, Tokyo, Japan
Andres, V:
Ctr Nacl Invest Cardiovasc, Dept Epidemiol Atherothrombosis & Imaging, Madrid, Spain
Martinez-Gonzalez, J:
IIB Sant Pau, Ctr Invest Cardiovasc CSIC ICCC, Barcelona, Spain
Minami, T:
Univ Tokyo, RCAST, Div Vasc Biol, Tokyo, Japan
Redondo, JM:
Ctr Nacl Invest Cardiovasc, Dept Vasc Biol & Inflammat, Madrid, Spain
Carlos III Hlth Inst, Inst Rare Dis Res, Dept Human Genet, Madrid, Spain
Campanero, MR:
CSIC UAM, Inst Invest Biomed Alberto So s, Dept Canc Biol, Madrid, Spain
Gold, Green Published, All Open Access, Green
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